| Specifications | |
|---|---|
| Organism/cell line/tissue | Human Coronary Artery Smooth Muscle Cells (HCASMC) |
| Sex | Male/female |
| Sequencer or array type | Illumina HiSeq2000 |
| Data format | Raw and analyzed |
| Experimental factors | siRNA treatment: siTCF21; siCONTROL |
| Experimental features | HCASMCs were purchased from Lonza and cultured according to the manufacturer's recommendations. Cells were serum-starved and transfected with siRNA pools from OriGene. Total RNA was collected and RiboZero libraries generated and sequenced on an Illumina HiSeq2000 instrument. The resulting reads were mapped to hg19 with TopHat [1]/Bowtie2 [2]. Differentially expressed genes were identified using either DESeq [3] and edgeR [4]. |
| Consent | Manufacturer's informed donor consent |
| Sample source location | n/a |
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