Figure 2.

Large GCs with a high frequency of apoptotic B cells in non-immunized BKO mice

1. IHC sections of naïve WT and BKO spleens stained for PNA and BCL6 expression.
2. Flow cytometry and number of B220⁺GL7⁺FAS⁺ GC B cells from WT (n = 8) and BKO (n = 11) spleens, along with percentage of YFP⁺ GC B cells, by flow cytometry, from WT-YFP (n = 8) and BKO-YFP (n = 8) spleens. ****P = 2.75E-05 and 1.3E-07 by Mann–Whitney U-test and two-tailed unpaired Student's t-test, respectively.
3. Flow cytometry for CD4, PD-1, CXCR5, and ICOS expression in splenocytes from WT-YFP (n = 5) and BKO-YFP (n = 5) mice. **P = 0.008 by Mann–Whitney U-test. qRT–PCR for Il21 expression, relative to 36b4 expression, in CD4⁺ splenic T cells from WT-YFP (n = 4) and BKO-YFP (n = 4) mice. **P = 0.0078 by two-tailed, unpaired Student's t-test.
4. Flow cytometry and number of B220⁺IgG1⁺ B cells from WT (n = 6) and BKO (n = 9) spleens. ***P = 0.0048 by Mann–Whitney U-test. qRT–PCR for Aicda expression, relative to 36b4 expression, from splenic B cells of WT (n = 4) and BKO (n = 4) mice. ***P = 0.0027 by two-tailed, unpaired Student's t-test.
5. Number of cleaved caspase 3⁺ TR, FO, MZ, and GC B-cell subsets from WT (n = 4) and BKO (n = 4) mice. *P = 0.029 by Mann–Whitney U-test.
6. Representative flow cytometry for B220, GL7, and NP expression in splenocytes from naïve BKO-YFP mice and LMCs immunized with NP-CGG 10 days post-immunization. Frequency of cleaved caspase 3⁺B220⁺GL7⁺ splenic cells (n = 4). ****P = 0.0005 by two-tailed, unpaired Student's t-test.

Data information: Mean ± s.d.