IHC sections of naïve WT and BKO spleens stained for PNA and BCL6 expression.
Flow cytometry and number of B220+GL7+FAS+ GC B cells from WT (n = 8) and BKO (n = 11) spleens, along with percentage of YFP+ GC B cells, by flow cytometry, from WT-YFP (n = 8) and BKO-YFP (n = 8) spleens. ****P = 2.75E-05 and 1.3E-07 by Mann–Whitney U-test and two-tailed unpaired Student's t-test, respectively.
Flow cytometry for CD4, PD-1, CXCR5, and ICOS expression in splenocytes from WT-YFP (n = 5) and BKO-YFP (n = 5) mice. **P = 0.008 by Mann–Whitney U-test. qRT–PCR for Il21 expression, relative to 36b4 expression, in CD4+ splenic T cells from WT-YFP (n = 4) and BKO-YFP (n = 4) mice. **P = 0.0078 by two-tailed, unpaired Student's t-test.
Flow cytometry and number of B220+IgG1+ B cells from WT (n = 6) and BKO (n = 9) spleens. ***P = 0.0048 by Mann–Whitney U-test. qRT–PCR for Aicda expression, relative to 36b4 expression, from splenic B cells of WT (n = 4) and BKO (n = 4) mice. ***P = 0.0027 by two-tailed, unpaired Student's t-test.
Number of cleaved caspase 3+ TR, FO, MZ, and GC B-cell subsets from WT (n = 4) and BKO (n = 4) mice. *P = 0.029 by Mann–Whitney U-test.
Representative flow cytometry for B220, GL7, and NP expression in splenocytes from naïve BKO-YFP mice and LMCs immunized with NP-CGG 10 days post-immunization. Frequency of cleaved caspase 3+B220+GL7+ splenic cells (n = 4). ****P = 0.0005 by two-tailed, unpaired Student's t-test.
Data information: Mean ± s.d.