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. 2015 Apr 27;16(6):753–768. doi: 10.15252/embr.201439505

Figure 5.

Figure 5

Loss of LKB1 induces IL-6 production through NF-κB activation
  1. qRT–PCR for Il6 expression, relative to 36b4 expression in WT, LKB1− (KO), and LKB1− reconstituted MEFs (KO + LKB1), in triplicate. Mean ± SEM; ***P = 0.0017 by one-way ANOVA.
  2. IL-6 secreted from WT, KO, and KO + LKB1 MEFs into culture media for 24 h, in triplicate. Mean ± SEM; ****P = 0.0001 by one-way ANOVA.
  3. Representative Western blot of WT, LKB1− (KO), and LKB1− reconstituted MEFs (KO + LKB1) for LKB1 and actin protein expression.
  4. Representative Western blot analysis of NF-κB family member proteins in nuclear lysates from WT, KO, and KO + LKB1 MEFs.
  5. qRT–PCR for Il6 expression, relative to 36b4 expression, in WT MEFS incubated with DMSO, and LKB1− MEFs incubated with either DMSO or 5 μM JSH-23. Mean ± s.d. for three independent experiments; **P = 0.006 by one-way ANOVA.
  6. Representative Western blot analysis of NF-κB family member proteins in nuclear lysates from CD43− B cells from WT, sorted YFP+, and sorted YFP− B cells from BKO mice (n = 3).