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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Nov 8;481(2):145–150. doi: 10.1016/j.abb.2008.11.001

Table 1. IC50 for SH-SY5Y neuroblastoma.

MTHFS expression diminishes the efficacy of LY309887. MTT Assay results represent average and standard deviation of three experiments. Trypan Blue Exclusion assay results are presented as the IC50 and the standard error of the mean from the concentration response curve as determined using Prism software with each measurement made in triplicate.

MTT Assay IC50 (nM) Trypan Blue Exclusion IC50 (nM)
SHSY-5Y SHSY-5YMTHFS SHSY-5Y SHSY-5YMTHFS
Pemetrexed 45 ± 20 33 ± 32 97 ± 27 114 ± 30
Lometrexol 223 ± 82 303 ± 107 490 ± 250 579 ± 448
LY309887 14 ± 2 29 ± 3* 46 ± 4 190 ± 24**
*

p=0.0002 comparing IC50 values in LY309887 for SH-SY5Y vs. SH-SY5YMTHFS using two-tailed, unpaired t-test.

**

p=0.03 comparing IC50 values in LY309887 for SH-SY5Y vs. SH-SY5YMTHFS using two-tailed, unpaired t-test.