Table 2. MTHFS activity in normal and tumor tissue biopsies.
The specific activities of MTHFS (in units of pmoles 5,10-methenylTHF/min/μg protein) were determined for the feline and canine tissue samples. Specific activity values shown represent the average of triplicate measurements and variation is expressed as standard deviations of the mean. The (*) indicates two independent biopsies from the same animal. For all tumors, one-tailed student's t-tests were performed assuming equal variance (p< 0.003).
| Normal Tissue | Tumor Tissue | Fold | ||
|---|---|---|---|---|
| Species | Tumor type | Specific Activity | Specific Activity | Increase |
| canine | mastocytoma | 0.07 ± 0.03 | 0.93 ± 0.01 | 13.30 |
| canine | mastocytoma | 0.08 ± 0.02 | 1.97 ± 0.08 | 24.63 |
| canine | mastocytoma | 0.18 ± 0.01 | 1.77 ± 0.09 | 12.64 |
| canine | mastocytoma | 0.33 ± 0.10 | 1.42 ± 0.04 | 4.30 |
| canine | mastocytoma | 0.06 ± 0.01 | 0.30 ± 0.01 | 5.00 |
| canine | sarcoma | 1.83 ± 0.08 | 3.38 ± 0.10 | 1.85 |
| canine | sarcoma | 0.36 ± 0.07 | 2.02 ± 0.02 | 5.61 |
| canine | sarcoma | 0.22 ± 0.01 | 1.47 ± 0.26 | 6.68 |
| feline | sarcoma | 0.09 ± 0.05 | 1.35 ± 0.04 | 15.00 |
| canine | melanoma | 1.43 ± 0.05 | 1.86 ± 0.09 | 1.31 |
| canine | osteosarcoma | 0.20 ± 0.03 | 1.29 ± 0.06 | 6.45 |
| canine | rectal carcinoma | n.d. | 0.03 ± 0.001 | \ |
| *feline | hemangiosarcoma | 0.57 ± 0.03 | 1.72 ± 0.13 | 3.02 |
| *feline | hemangiosarcoma | 0.49 ± 0.04 | 3.43 ± 0.10 | 7.00 |
| feline | breast | n.d. | 0.12 ± 0.01 | \ |