Table 2.

Maximal concentrations of cytokines produced in culture supematants from NEQ-48-treated lymphocyte cultures.

NBQ-48, μg/mL	Cytokine	Cytokine concentration; pg/mL±SEM
0.6	G-CSF	67.6±57.5
2.5	IL-2	37.2±15.1
5.0	IL-6	65.2±40.2
0.6	IFN-γ	3.8±3.8

Murine splenic lymphocytes were isolated and incubated with twofold serial dilutions of NBQ-48 (5-0.3 μg/mL) for 5 days. Culture supernatants were collected and the cytokine concentrations were determined ay protein microarray analysis. Supernatants from Concanavalin A-treated lymphocytes, and media alone were used as positive and negative controls, respectively. Results were expressed as mean concentrations ± SEM of three independent experiments.