Fig. 2.

(A and B) Time courses of NREM and REM sleep, changes in NREM sleep delta power and body temperature in EP₃ flox/Nes-Cre (A), EP₄ flox/Nes-Cre (B) mice and their control littermates (EP₃ flox and EP₄ flox, respectively) after LPS administration (15 µg/mouse 30 min before dark onset) during the 12-h light and dark periods. Percent change of NREM sleep delta power was calculated based on the average of the NREM sleep delta power during 24 h under the baseline condition. Data are presented as the mean ± SEM (A, n = 3–5, and B, n = 5–7). *p < 0.05, **p < 0.01 compared to saline injection, as assessed by paired two-tailed Student’s t tests. Black and white bars above the x-axes indicate the dark and light periods. (C) Total amount of NREM sleep during the dark period after LPS or saline injection in NS-specific KO mice for EP₃ and EP₄ receptors and their control littermates (EP₃ flox and EP₄ flox, respectively) as well as total body KO mice for the microsomal PGE synthase-1 and PGD₂ receptors. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01 compared to saline injection, as assessed by paired two-tailed Student’s t tests.