The effect of ECG on neuronal organization was assessed in Tsc1GFAPCKO mice by cresyl violet staining. Compared with control mice (A), vehicle-treated Tsc1GFAPCKO mice (B) exhibited widely dispersed pyramidal cell layers (arrows) in all regions of hippocampus (CA1–CA4). ECG treated Tsc1GFAPCKO mice (C) had a similar pattern as vehicle-treated Tsc1GFAPCKO group (B), with no apparent effect on this neuronal disorganization. Scale bar = 500 μm. Cont = control mice, KO = Tsc1GFAPCKO mice, Veh = vehicle, ECG = Epicatechin-3-gallate.