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. 2015 Jun 15;10(6):e0130055. doi: 10.1371/journal.pone.0130055

Fig 5. Effects of KL on the NO release and expression of target proteins measured by western blotting and immunofluorescence analysis.

Fig 5

(A) Immunoblots of brain hippocampus homogenates with antibodies specific for 5-HT1AR, p-CREB, CREB, p-PKA, PKA, and nNOS. (B) Effects of KL on the release of NO in the hippocampus. *, **: different from the NO content of the blank group, P < 0.05, P < 0.001; ##: different from the NO content of the control group, P < 0.001; n = 12, mean ± SD; Bla: blank group, Con: control group, Bus: buspirone group, KL: Kadsura longipedunculata group. (C) Confocal immunofluorescence analysis of rat hippocampus with 5-HT1AR mAb (red) and neuro trace (green). The neuro trace was used to identify neurons.