Fig 1. Schematic illustration of the AAV vector used to transfer the HBV genome and in vitro determination of the replication ability of the construct.

(a) A fragment containing 1.2 copies of the HBV genome (genotype D) was generated from the pHBV1.2 plasmid and cloned into the p-SSV9 vector, which contained the ITR of AAV type 2 at both ends by exchanging the rep and cap genes (see Materials and Methods). The q-PCR primers were indicated. (b, c) Plasmid of pSSV9-1.2HBV (3μg) was transfected to Huh7.5.1 cell and determined the HBV viral genomes contents and/or the cumulative expression of HBsA and HBeAg in the cell and/or supernatant respectively, by Q-PCR and ELISA. Data represent the mean ±SD (n = 4).