Fig 4. DSB-mediated RNA induction and telomerase inhibition are associated with DRE.

(A) Table indicating the TER2 transcript status for four A. thaliana accessions. (B) and (C) show qPCR results for Col-0 and Ler-0 seedlings treated with zeocin for the time points indicated. Data for the BRCA1 control (B) and TER2 (Col-0) or TER2Δ (Ler-0) transcripts (C) are shown. (D) qPCR results for accessions with TER2 (Ws-2) and TER2Δ (Co-1) submitted to the zeocin regimen for 2 h. (E) qPCR results following TERT immunoprecipitation in Col-0 and Ler-0 seedlings treated with or without zeocin (time point). The TER2:TER1 ratio in Col-0 and the TER2Δ:TER1 ratio in Ler-0 are shown. Values were normalized to Col-0 TER2:TER1 ratio in the absence of zeocin (set to 1). (F) qTRAP results for Col-0 and Ler-0 seedlings with or without zeocin treatment. (G) qTRAP results for the samples in (D) and the 2 h time point from (C). Telomerase activity was normalized to the corresponding untreated controls and set to 1. Red dashed bar indicates no change between treated and untreated samples. The changes in telomerase activity in Col-0 and Ws-2 were statistically significant (p-value< 0.05). Significance was calculated relative to untreated samples using a Student’s t-test. For all experiments, n > 3.