Fig 6. TER2 is a labile RNA transcript stabilized by DNA damage.
qPCR results are shown for TER1 and TER2/TER2Δ from Col-0 and Ler-0 in the presence of cordycepin. Col-0 and Ler-0 seedlings were treated with cordycepin (100μg/μl) for the times indicated followed by qPCR to monitor TER1 (A) and TER2/ TER2Δ (B). The values obtained for untreated RNA samples were set to 0 and the fold decrease is shown. eIF-4a was used as reference gene for normalization. (C-E) qPCR results from a time course experiment of Col-0 seedlings treated with cordycepin followed by zeocin. Seedlings were incubated with cordycepin for 1.5 h to shut down transcription, and zeocin was added (red arrows). The incubation continued for a total of 3.5 h. Results for BRCA1 (C), TER1 (D), TER2 (E) are shown.