1 |
Not contain the adaptor/acceptor in sequencing sequences. |
2 |
Sequences length between 17–25 nt. |
3 |
No-exceed 80% base A, or G, or C, or T. |
4 |
Not match the mRNAs, rRNA and tRNA. |
3 |
Minimal Free energy (dG in kCal/mol) ≤ -15. |
5 |
Minimal folding free energy index (MEFI) ≥ 0.7 *. |
6 |
Number of allowed errors in one bulge in stem ≤ 12. |
7 |
Number of base pair in stem region ≥ 16. |
8 |
Length of hairpin which the up and down stem plus terminal region ≥ 50 nt. |
9 |
Length of terminal loop ≤ 350 base pair. |
10 |
Number of allowed errors in one bulge in mature region ≤ 8. |
11 |
Number of allowed biased errors in one bulge in mature region ≤ 4. |
12 |
Number of allowed biased bulges in mature region ≤ 2. |
13 |
Number of base pair (nt) in mature or mature* region ≥ 12. |
14 |
Percentage of small RNA in stem region (pm) ≥ 80%. |
15 |
Number of allowed errors in mature region ≤ 7. |