| 1 |
Not contain the adaptor/acceptor in sequencing sequences. |
| 2 |
Sequences length between 17–25 nt. |
| 3 |
No-exceed 80% base A, or G, or C, or T. |
| 4 |
Not match the mRNAs, rRNA and tRNA. |
| 3 |
Minimal Free energy (dG in kCal/mol) ≤ -15. |
| 5 |
Minimal folding free energy index (MEFI) ≥ 0.7 *. |
| 6 |
Number of allowed errors in one bulge in stem ≤ 12. |
| 7 |
Number of base pair in stem region ≥ 16. |
| 8 |
Length of hairpin which the up and down stem plus terminal region ≥ 50 nt. |
| 9 |
Length of terminal loop ≤ 350 base pair. |
| 10 |
Number of allowed errors in one bulge in mature region ≤ 8. |
| 11 |
Number of allowed biased errors in one bulge in mature region ≤ 4. |
| 12 |
Number of allowed biased bulges in mature region ≤ 2. |
| 13 |
Number of base pair (nt) in mature or mature* region ≥ 12. |
| 14 |
Percentage of small RNA in stem region (pm) ≥ 80%. |
| 15 |
Number of allowed errors in mature region ≤ 7. |
