Table 2. mCherry Core Reversion Variants.
| Mutation | Brightnes (% mCherry) | Apparent Tm (% mCherry) | Shift in emission (nm) |
|---|---|---|---|
| WT mCherry | 100 | 100 | — |
| A44V | 93 | 96 | +2 |
| M68Q | 90 | 95 | -3 |
| A71V | 100 | 100 | -3 |
| L124F | 99 | 99 | — |
| M150L | 91 | 98 | -1 |
| Q163K | 108 | 98 | — |
| A175V | 84 | 98 | — |
| V177F | 24 | 93 | +4 |
| T179S | 95 | 98 | +1 |
| A217T | 104 | 91 | -2 |
| M150L / Q163K | 104 | 99 | -2 |
| Q163K / T179S | 94 | 99 | +1 |
| A71V / Q163K / T179S | 100 | 99 | -1 |
| A71V / M150L / Q163K / T179S | 95 | 98 | -3 |
| A71V / L124F / M150L / Q163K / T179S | 93 | 97 | -3 |
| A44V / A71V / L124F / M150L / Q163K / T179S (mCherryR6) | 81 | 89 | -1 |
Peak emission wavelength, brightness, and thermostability resulting from reversion mutations to mCherry’s core.