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. 2015 Jun 15;10(6):e0129643. doi: 10.1371/journal.pone.0129643

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — Ovaries at 17.5 dpc were cultured for 7 days without treatment (as a control) or with 100 ng/mL ACT A. Compared to control ovaries (A, black boundary enlarged in B), there were significantly more primordial follicles in the periphery of ovaries treated with ACT A (C, black boundary enlarged in D). The total number of ovarian follicles in the largest cross-section of control and ACT A-treated ovaries were quantified (E). Western blot analysis revealed an obvious increase of the p-smad2 level in Activin A-treated ovaries relative to control group (F). Scale bars: 40 μm (A-D). Smad2 was measured as internal control. Significant differences are indicated by * (P < 0.05).