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. 2015 Jun 15;10(6):e0129786. doi: 10.1371/journal.pone.0129786

Fig 6. In vitro characteristics of NCF1*/* and NCF1*/+ MCA induced tumors.

Fig 6

Tumors cell lines were generated from MCA induced tumors by mechanical dissociation followed by treatment with Collagenase IV and Trypsin. Tumor cells were plated at low confluence on Xcelleigence E-plates and (Ai) growth was monitored over time and (Aii) cell growth rate was determined based on the logarithmic growth phase. (Bi) Tumor cells were seeded onto CIM-16 xCELLigence plates with serum medium gradient separated by matrigel plug to evaluate invasion for which the (Bii) invasion rate was compared between NCF1*/* and NCF1*/+ cell lines. (C) Tumor cells were labeled with DHR-123 and analyzed by flow cytometry.