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. 2014 Jul 9;34(28):9404–9417. doi: 10.1523/JNEUROSCI.1771-14.2014

Figure 5.

Figure 5.

5-HT presynaptic inputs to PPNs. A, An immunostaining image of wild-type mouse spinal cord section with 5-HT antibody. B, A diagram of 5-HT axons in the spinal cord. C, Immunostaining of a thoracic spinal section of a wild-type mouse with antibodies against GFP (rabies-GFP), 5-HT, and NeuN together with DAPI labeling. The boxed area highlights the 5-HT contact with the PPN, and the inset is the close-up view of the bouton. D, A dot map represents the distribution patterns of thoracic PPNs contacted (red square) and not contacted (black square) by 5-HT axons. E, An image of a wild-type mouse cervical spinal section immunostained with antibodies against GFP (rabies-GFP) and 5-HT showing a rabies-infected cervical PPN contacted by two 5-HT boutons. A high-magnification image of the boutons is shown in the inset. The spinal section is counterstained with NeuN and DAPI. F, The distribution of cervical PPNs with (red squares) or without (black squares) 5-HT boutons in the cervical spinal cord. Scale bars: A, 100 μm; C, 20 μm; E, 15 μm.