Fig 1. In vitro-produced (IVP) blastocysts are characterized by abnormal actin cytoskeleton organization.

(A) Overview of the experimental design. Blastocysts were collected after either mating and in vivo development (IVO group) or in vitro fertilization and culture (IVP group). (B) Protein-protein interaction (PPI) network. Differentially expressed genes (DEGs) in the IVO and IVP blastocysts showed a tightly interconnected network, as observed from a web-based search of the STRING database. (C) Expression of actin-associated genes in the IVP and IVO blastocysts detected by quantitative real-time PCR analysis. The asterisks (* or **) in each column represent statistically significant differences (P < 0.05 and P < 0.01, respectively).