Fig 3. Immunolocalization of nuclei after staining with DAPI of control, T4- and DHT-treated cysticerci.
Phase contrast of the control sections of the slides obtained by Tissue-Teck fixing are shown in upper panel from left to right, control (C), testosterone (T4) and dihydrotestosterone (DHT). In lower panel, DAPI was used to detect nuclei in frozen tissue sections fixed with Tissue-Teck. Treatment with both steroids induced changes in the distribution of nuclei, which also affected the morphology of FCs. Cysticerci were observed under an SEM Zeiss DSM-950 after five days of treatment with 50 μg/ml of each steroid. Scale bar corresponds to 10 μm.