Fig 4. Immunolocalization of α-tubulin (green) of control, T₄ and DHT treated parasites.

Phase contrast of the control sections of the slides obtained by Tissue-Teck fixing are shown in upper panel from left to right, control (C), testosterone (T₄) and dihydrotestosterone (DHT). In lower panel, green tiny spots are produced by the specific binding of the anti-α-tubulin stained with alexa-488 antibody to cytoskeletal α-tubulin. Tubulin protein inside the bladder wall and at the level of the tegument in the tissue of control parasites and those treated with T₄ and DHT. Scale bar corresponds to 10 μm.