Table 4. qRT-PCR validation of selected gene.
Genes | Fold-change TINT vs. RPMI control (microarray) | p-value | Fold-change TINT vs. RPMI control (qRT-PCR) | p-value | Fold-change heat-killed AT-1 vs. RPMI control (qRT-PCR) | p-value |
---|---|---|---|---|---|---|
Lox | 5.2 | 0.001 | 3.4 | 0.021 | -1.9 | 0.043 |
Hmox1 | 14.5 | 0.001 | 50.9 | 0.000 | -1.2 | 0.290 |
Cd68 | 4.9 | 0.007 | 11.3 | 0.007 | -1.1 | 0.211 |
Lpl | 5.3 | 0.027 | -1.7 | 0.630 | -1.2 | 0.749 |
Cebp-beta | 2.3 | 0.009 | 7.8 | 0.001 | -2.6 | 0.008 |
Cyr61 | 2.2 | 0.010 | 5.4 | 0.002 | 1.6 | 0.298 |
Mmp3 | 2.6 | 0.017 | 5.1 | 0.003 | -3.4 | 0.013 |
S100a4 | 5.2 | 0.003 | 20.2 | 0.002 | -1.2 | 0.036 |
Tgf-beta | 2.4 | 0.008 | 31.3 | 0.003 | -1.3 | 0.037 |
Mme | -2.0 | 0.009 | -1.7 | 0.211 | -1.5 | 0.012 |
Gstm1 | -2.0 | 0.001 | -1.5 | 0.016 | 1.0 | 0.923 |