Fig 4. Reproducibility of DENV RT-RPA assays.

A, DENV1-3 and B, DENV4 RT-RPA assays. Semi-logarithmic regression of the data collected from eight DENV RT-RPA test runs on the RNA standard using PRISM. Both assays yielded results between 3–7 minutes. In DENV1-3 RT-RPA assay, 10⁷–10³ RNA molecules were detected 8 out of 8 runs, 10² in 1 out of 8 and 10 copies was not identified. In DENV4 RT-RPA assay, 10⁷–10² RNA molecules were detected 8 out of 8 runs and 10 copies in 6/ out of 8. In Fig 4B, the value for 10 RNA copies was consistently 5.3 minutes in all 6 cases.