Table 2. Sequence of primers and probes for the construction of molecular standards, real-time RT-PCR and RT-RPA assays.
| Name | Sequence 5’-3’ | Amplicon size bp |
|---|---|---|
| DENV1-3-STD-UP | CTGTACGCACGGTGTAGCAGAC | 242 |
| DENV1-3-STD-DP | CCTGTTGATTCAACAGCACCATTC | |
| DENV4-STD-UP | CTGTACGCGTGGCATATTG | 248 |
| DENV4-STD-DP | CCTGTTGGATCAACAACACC | |
| DENV-PCR-FP | AAGGACTAGAGGTTAKAGGAGACCC | 85 |
| DENV-PCR-RP | CTGHRGAGACAGCAGGATCTCTG | |
| DENV-PCR-P | FAM-AACAGCATATTGACGCTGGGARAGAC-TAMRA | |
| DENV1-3-RPA-FP13 | ATTCAACAGCACCATTCCATTTTCTGGCGTTCTGTG | 97 |
| DENV1-3-RPA-RP4 | AACAGCATATTGACGCTGGGAGAGACCAGAGATC | |
| DENV4-RPA-FP3 | CATCTTGCGGCGCTCTGTGCCTGGATTGA | 86 |
| DENV4-RPA-RP2 | CACAAAAACAGCATATTGACGCTGGGAAAG | |
| DENV-RPA-P3 | ATATTGACGCTGGGAGAGACCAGAGATCCTGC(BHQ1-dT)(THF)(FAM-dT)CTCCTCAGCATCATTC-(Phosphate) | N/A |
Bp, base pair; DENV1-3-STD-UP/DP are for DENV1-3 RT-PCR; DENV4-STD-UP/DP are for DENV4 RT-PCR; DENV-PCR-FP/RP/P, real-time RT-PCR primers and Taqman probe (FAM/TAMRA); DENV1-3-RPA-FP, forward and reverse primers for DENV1-3 RT-RPA; DENV4-RPA-FP, forward and reverse primers for DENV4 RT-RPA; DENV-RPA-P, exo-probe; BHQ1-dT: thymidine nucleotide carrying Blackhole quencher1, THF: tetrahydrofuran spacer, FAM-dT: thymidine nucleotide carrying Fluorescein. N/A, non applicable.