Figure 2. Effect of miR-148a expression on growth, clonogenic potential and, anchorage independent growth of medulloblastoma cell lines studied using stable polyclonal populations (P1, P2) of Daoy and D425 cells expressing miR-148a on doxycycline induction.

(A) Y axis denotes percentage growth inhibition obtained on doxycycline induction of the vector control and P1, P2 polyclonal populations of the indicated cell line as judged by the MTT assay. (B) Y axis indicates percent reduction in the number of colonies formed on doxycycline induction of the vector control and P1, P2 population cells of Daoy cells and percent reduction in the number of colonies formed on irradiation of doxycycline induced indicated population, in a clonogenic assay. (C, D) Y axis denotes the number of colonies formed in a soft agar assay by the vector control and P1, P2 population of the indicated cell line. The significance of the difference in the growth inhibition or soft agar colony formation observed on doxycycline induction of the polyclonal populations expressing pTRIPZ-miR-148a as compared to that observed in the polyclonal population transfected with control pTRIPZ vector was calculated by the student's t test. ** indicates p < 0.001 while *** indicates p < 0.0001 as determined using Student's t test.