Figure 5. DAXX expression is inversely correlated with the expression of positive regulators of autophagy.

Using RNA-Seq as described in Figure 4, among autophagy genes, DAXX expression was inversely correlated with expression of positive regulators of autophagy, including DAPK1, DAPK3, ATG8, ATG18, ATG101, LC20, ATG3, ATG4, Beclin1, ATG7, and Deptor (not shown), whereas it was linearly correlated with negative regulators of autophagy mTOR and Raptor (Figure 6). Duplicate samples were used per cell line. The graphs were prepared by determining gene expression using HOMER, and then plotted using Excel.