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. 2015 May 14;2(5):542–554. doi: 10.18632/oncoscience.162

Figure 2. Effect of PBMC lysate dilution on protein quantitation.

Figure 2

Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.