Table 1. Intra-assay Imprecision.
| Sample ID | Analyte | Mean Signal Intensity (RFU × 105) | cv (%) | n | Ratio (phospho/pan protein) | cv (phospho/pan protein) (%) | Fold-Activation |
|---|---|---|---|---|---|---|---|
| Control A | phospho-ATM | 4.64 ± 0.24 | 5.1% | 8 | 0.07 ± 0.003 | 4.7% | |
| Control B | phospho-ATM | 3.49 ± 0.17 | 4.9% | 8 | 0.12 ± 0.008 | 6.2% | |
| Control A | pan-ATM | 64.3 ± 1.16 | 1.8% | 8 | |||
| Control B | pan-ATM | 28.2 ± 1.26 | 4.5% | 8 | |||
| 2 Gy IR A | phospho-ATM | 47.9 ± 1.94 | 4.0% | 8 | 0.7 ± 0.036 | 4.9% | 10.38 ± 0.81 |
| 2 Gy IR B | phospho-ATM | 57.8 ± 0.92 | 1.6% | 8 | 1.26 ± 0.035 | 2.8% | 10.21 ± 0.80 |
| 2 Gy IR A | pan-ATM | 64.2 ± 2.09 | 3.3% | 8 | |||
| 2 Gy IR B | pan-ATM | 45.9 ± 1.39 | 3.0% | 8 | |||
| Control A | γH2AX | 45.7 ± 0.69 | 1.5% | 8 | 8.37 ± 0.50 | 6.0% | |
| Control B | γH2AX | 20.1 ± 1.20 | 6.0% | 8 | 8.21 ± 0.87 | 10.6% | |
| Control A | pan-H2AX | 5.42 ± 0.29 | 5.3% | 8 | |||
| Control B | pan-H2AX | 2.47 ± 0.21 | 8.6% | 8 | |||
| 2 Gy IR A | γH2AX | 165 ± 2.70 | 1.6% | 8 | 29.60 ± 0.83 | 2.8% | 3.55 ± 0.19 |
| 2 Gy IR B | γH2AX | 163 ± 6.59 | 4.0% | 8 | 29.91 ± 1.48 | 5.0% | 3.68 ± 0.41 |
| 2 Gy IR A | pan-H2AX | 5.58 ± 0.18 | 3.2% | 8 | |||
| 2 Gy IR B | pan-H2AX | 5.47 ± 0.18 | 3.3% | 8 |