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. 2015 May 19;2(5):555–566. doi: 10.18632/oncoscience.166

Figure 1. TALEN mediated targeting of the MCR of the Xenopus tropicalis apc gene.

Figure 1

(A) Schematic representation of the apc protein, the region that was targeted by TALENs, and the TALENs used. (B) Deletions detected in the targeted region of apc gene in embryos injected with 300 pg of apc TALEN mRNA. The sequences in red indicate the apc TALEN recognition sites. (C) Bi-allelic mutation of the apc gene identified in desmoid and hindlimb tumors dissected from tadpoles having undergone apc TALEN mRNA (40 pg) injection. AA, Amino Acid; APC, Adenomatous Polyposis Coli; Arm, Armadillo repeats; MCR, Mutation Cluster Region; EB1 BD, End Binding protein 1 Binding Domain; HD, Homodimerization domain; MT BD, Microtubule Binding Domain.