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. 2015 May 27;17(5):401–409. doi: 10.1016/j.neo.2015.04.001

Figure 5.

Figure 5

(A) Real-time PCR expression of VCAM-1, vWF, and ICAM-1 RNA in MDS ECFCs incubated with Wnt3A, Wnt5A, and Wnt3A plus Wnt5A (each at 100 ng/ml concentration). The data represent the mean values of three experiments (SDs are < 10% and have been omitted). (B and C) The flow cytometry expression of ICAM-1 in normal (gray line) and MDS (red line) ECFCs without Wnt3A (B) and after incubation with Wnt3A (C). We observed a significant reduction of ICAM-1 in MDS ECFCs. Data refer to a representative experiment; mean fluorescence intensity is reported in the x axis and number of events in the y axis. (D) Co-cultures of normal cord blood CD34 + cells with normal ECFCs (white columns), MDS ECFCs (red columns), or MDS ECFCs plus 100 ng/ml Wnt3A (gray columns). Real-time PCR expression of TRF, MPO, and GPIb RNA after day 7 of culture in erythroid, granulo-monocytic, and megakaryocytic differentiating media. Results represent the mean of five experiments. *P < .05; ***P < .001.