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. 2015 May 27;17(5):421–433. doi: 10.1016/j.neo.2015.04.003

Figure 1.

Figure 1

The tumor stroma of the MMTV-Neu and C3(1)-Tag mouse models of breast cancer is different. (A) Representative images of mammary tumors at different stages from MMTV-Neu and C3(1)-Tag mice. Tumors were stained with hematoxylin and Masson’s trichrome for visualization of collagen (which stains blue). Black arrows indicate deposition of collagen in the tumor microenvironment. Scale bars, 100 μm. (B) Myeloid cell infiltration in carcinomas from MMTV-Neu and C3(1)-Tag mice. The tumors were stained for reactivity against antigen Ly6B.2 (clone 7/4, expressed on neutrophils/monocytes) and F4/80 (expressed on macrophages). Red arrows indicate positively stained cells. Scale bars, 100 μm. (C) Significantly more neutrophils and macrophages were detected in tumors from C3(1)-Tag (n = 5) than MMTV-Neu (n = 3 for macrophage analysis and n = 5 for neutrophil analysis) mice (P = .008 and P = .04, respectively, Mann-Whitney, two-sided). (D) Relative protein levels of a panel of chemokines/cytokines measured on whole-tumor lysates from MMTV-Neu (n = 4) and C3(1)-Tag (n = 4) tumors. Each column represents a tumor from an individual mouse. The measured protein levels were normalized to an internal assay standard and plotted in a heat map. Statistical significance was determined by t tests using the Holm- Šídák method to correct for multiple comparisons, and the asterisk indicates P < .05.