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. 2015 May 27;17(5):421–433. doi: 10.1016/j.neo.2015.04.003

Figure 6.

Figure 6

The absence of MMP9 increases IGFBP-1 protein and suppresses the IGF signaling pathway. (A) Relative protein levels of a panel of angiogenic factors in C3(1)-Tag;Mmp9+/+ versus C3(1)-Tag;Mmp9−/− and (B) MMTV-Neu;Mmp9+/+ versus MMTV-Neu;Mmp9−/− tumors. Each column represents a tumor from an individual mouse. The measured protein levels were normalized to an internal assay standard and plotted in a heat map. Statistical significance was determined by t tests using the Holm-Šídák method to correct for multiple comparisons, and the asterisk indicates P < .05. Only MMP9 protein levels were significantly different after correction for multiple comparisons. (C) Representative images of immunohistochemistry with antibodies against IGFBP-1 and (E) phospho IGF-1R on tumor sections from C3(1)-Tag;Mmp9+/+ (n = 10) and C3(1)-Tag;Mmp9−/− (n = 10) mice. Positive staining is shown in brown; slides were counterstained with hematoxylin. Quantification of the (D) increase in IGFBP-1 protein (P = .05, t test) and (F) decrease in phosphorylated IGF-1R protein (P = .002, t test) in tumors of C3(1)-Tag;Mmp9−/− mice. The H-score was determined by ImageScope software after scanning the stained slides and was calculated as the sum of three times the area of strong staining intensity and two times the area of medium staining intensity. Scale bar, 100 μm.