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. 2015 Jun 16;6:394. doi: 10.3389/fpls.2015.00394

FIGURE 4.

FIGURE 4

Expression of the AtTZF1-5, TdTZF1-A, and TdTZF1-B genes during the germination process. Arabidopsis and T. durum seeds were germinated in water or in the presence of 150 mM NaCl. The relative expression levels are shown for AtTZF1-5, TdTZF1-A, and TdTZF1-B in germinating seeds in water and in NaCl solution after 6 h and 12 h from the onset of germination. The relative expression levels are also shown for TdTZF1-A and TdTZF1-B in stratified seeds. Relative mRNA expression levels were referred to the dry seeds. Data are means of relative quantification (Log2) of three biological replicates normalized to AtTIP41-like and Td_17S1 for the A. thaliana and T. durum target genes, respectively (±SE). Statistical significance was assessed by one-way ANOVA analysis followed by Bonferroni’s tests. °P < 0.05, ∘∘P < 0.01 72 h strat., 6 h H2O, 12 h H2O vs dry seeds; ∗P < 0.05, ∗∗P < 0.01 6 h NaCl vs 6 h H2O, 12 h NaCl vs 12 h H2O.