Table 1.

Presence of type III secretion systems, type III effectors, hrp/hpa regulatory genes, and homologs of predicted HrpX regulon members of X. cannabis pv. cannabis in representative strains of Xanthomonas.

Group	Species	Strain	GenBank accession no.	Hrp T3SS	T3Es	HrpG HrpX	HpaS HpaR2	PehA	PehD	Amino-peptidase	LPL
1	X. albilineans	GPE PC73	FP565176	no	no	no	YES	YES	YES	no	YES
1	X. sacchari	NCPPB 4393	AGDB01000001	no	no	no	YES	YES	no	YES	YES
1	X. translucens pv. cerealis	CFBP 2541	JWHD01000001	YES	YES	YES	noa	YES	YES	Ψ	YES
	X. maliensis	97M	AQPR01000001	no	no	YES	YES	no	PIP	PIP[5]	PIP[1]
2	X. campestris pv. campestris	ATCC 33913	AE008922	YES	YES	YES	YES	PIP	PIP[1]	PIP[1]	PIP
2	X. vesicatoria	ATCC 35937	AEQV01000004	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. vasicola pv. vasculorum	NCPPB 206	AKBM01000001	YES	YES	YES	YES	PIP	no	PIP[1]	PIP
2	X. oryzae pv. oryzae	KACC110331	AE013598	YES	YES	YES	nob	Ψ	YES	no	PIP
2	X. fuscans subsp. fuscans	4834-R	FO681494	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. citri pv. citri	306	AE008923	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. axonopodis pv. manihotis	CIO1	AKCZ01000001	YES	YES	YES	YES	PIP	no	PIP[1]	PIP-Ψ
2	X. axonopodis pv. vasculorum	NCPPB 900	JPHD01000001	YES	YES	YES	noc	PIP	PIP	PIP[1]	PIP
2	X. euvesicatoria	85-10	AM039952	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. cassavae	CFBP 4642	ATMC01000001	YES	YES	YES	YES	PIP	PIP	PIP[2]	PIP[3]
2	X. arboricola pv. celebensis	NCPPB 1832	JPHC01000001	YES	YES	YES	YES	PIP	no	PIP[1]	PIP[1]
2	X. hortorum pv. carotae	M081	AEEU01000001	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. gardneri	ATCC 19865	AEQX01000001	YES	YES	YES	YES	PIP	PIP	PIP[1]	PIP
2	X. fragariae	LMG 25863	AJRZ01000001	YES	YES	YES	YES	PIP	PIP	Ψ	Ψ
2	X. cannabis pv. phaseoli	Nyagatare	JRQI01000001	YES	YES	YES	YES	PIP	PIP	PIP	PIP
2	X. cannabis pv. cannabis	NCPPB 2877	JSZE01000001	no	no	YES	YES	PIP	PIP	PIP	PIP
2	X. cannabis pv. cannabis	NCPPB 3753	JSZF01000001	no	no	YES	YES	PIP	PIP	PIP	PIP

“YES” indicates presence of a homolog, “no” indicates absence of the protein(s).

“Ψ” indicates pseudogenes, i.e., the gene is split into two or more fragments.

“PIP” indices the presence of the gene along with a canonical PIP box and a properly spaced −10 promoter motif. Numbers in square brackets indicate the number of single-nucleotide variants with respect to the canonical PIP box and −10 promoter motif.

^ahpaR2 is eroded while an N-terminally truncated form of hpaS is present in X. translucens pv. cerealis strain CFBP 2541. Remnants are found in two other X. translucens strains (ART-Xtg27 and DSM 18974). Interestingly, the hpaR2/hpaS locus is intact in X. translucens strain DAR61454. This is an example of ongoing gene erosion in one species of Xanthomonas.

^bThe hpaR2/hpaS locus got destroyed by an IS element in X. oryzae pv. oryzae strain KACC 10331. Yet, hpaR2/hpaS is present in X. oryzae pv. oryzicola strain BLS256. Remnants are found in the African X. oryzae pv. oryzae strain NAI8 and in X. oryzae strains from the United States. This finding illustrates ongoing gene erosion in another species of Xanthomonas.

^chpaR2 is absent while an N-terminally truncated form of hpaS is present in X. axonopodis pv. vasculorum strain NCPPB 900.