Figure 3.
Length of the transcriptional memory in trained plants. The relative levels of mRNA of acclimated plants after recovery period under high temperature conditions for 2, 4, or 8 days, before and after a subsequent heat stress are shown in (A–H). Plants were not stressed (CK), were heat stressed after high temperature pre-acclimation (PA) or without acclimation (NA). Transcript levels of the trainable (HMW-HSP and LMW-HSP) genes. Transcript levels of the non-trainable (HSC 70 and OsHSP 74.8) genes measured by reverse transcription–quantitative PCR (qPCR) in the leaves of PI 574522 (A–D) and PI 512315 (E–H) at 2, 4, and 8 days of heat stress. YT-521B was used as an internal control.