TABLE 3.
Residual glucuronidation activity after treatment with inhibitors
Control incubations contained vehicle only (DMSO for diclofenac and sulfynpirazone, and methanol for hecogenin). Data represent means ± S.D. of duplicate determinations in a single experiment.
| UGTs | Residual Activity (% Control) |
||
|---|---|---|---|
| 1 mM Sulfinpyrazone | 200 µM Hecogenin | 500 µM Diclofenac | |
| UGT1A1 | N.A. | N.A. | 14.5 ± 0.9 |
| UGT1A3 | 65.9 ± 0.4 | N.A. | N.A. |
| UGT1A4 | N.A. | 0 | N.A. |
| UGT1A6 | N.A. | N.A. | 11.7 ± 0.9 |
| UGT1A7 | N.A. | N.A. | 0 |
| UGT1A8 | N.A. | N.A. | 0 |
| UGT1A9 | N.A. | N.A. | 20.3 ± 0.1 |
| UGT1A10 | N.A. | N.A. | 0 |
| UGT2B4 | N.A. | N.A. | 0 |
| UGT2B7 | N.A. | N.A. | 4.5 ± 0.0 |
| UGT2B15 | N.A. | N.A. | 8.5 ± 0.1 |
| UGT2B17 | N.A. | N.A. | 0 |
N.A., not applicable.