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. 2015 Jul;43(7):1084–1090. doi: 10.1124/dmd.115.063628

Fig. 3.

Fig. 3.

Time course for the activation of IFO and d4IFO by CYP3A4. IFO (blue) or d4IFO (red) (0.1–5.0 mM) was incubated in the standard reconstituted system containing 1 μM CYP3A4 for 30 minutes at 37°C. The details of the analytical methods are provided in the Materials and Methods. Shown are the nonlinear fits for the activation of the drugs determined using the Michaelis-Menten equation with Prism 6 (GraphPad Software, Inc.). The Km and Vmax values for all of the P450s tested are summarized in Table 1.