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. 2015 Apr 22;89(13):6918–6927. doi: 10.1128/JVI.00332-15

FIG 4.

FIG 4

Viral and host determinants of SIV sensing by pDC. (A and B) PBMCs from AGM (n = 5 to 23) (A) and Chinese rhesus MAC (n = 2 to 15) (B) were stimulated with medium (Mock), five SIVagm strains, three SIVmac strains, or HIV-1 (HIV-1.bal) at three concentrations: 1,500 ng/ml, 150 ng/ml, and 15 ng/ml p24/p27. Median values are represented by a horizontal line (blue and red for SIVagm and HIV/SIVmac, respectively). *, Wilcoxon, P < 0.05; **, Wilcoxon, P < 0.01 (compared to stimulation with an equal dose of SIVagm.sab92018). nd = not determined. tox = cytotoxic in 18-h culture. (C) AGM PBMCs (n = 10) were stimulated with medium (gray circle) or with 15, 150, or 1,500 ng/ml p27 SIVmac251 (red square) or SIVmac239 (blue triangle) for 18 h, after which viability was measured. Median and interquartile ranges are depicted. *, Wilcoxon, P < 0.05. Neg. Ctrl, negative control. (D) AGM PBMCs were stimulated with HIV (n = 14), VSV-G-pseudotyped HIV (n = 10), SIVmac251 (n = 10), VSV-G-pseudotyped SIVmac251 (n = 9), or SIVagm.sab92018 (n = 23) at 150 ng/ml p24 or p27. Median values and interquartile ranges are represented by a line and bars, respectively. *, Kruskal-Wallis, P < 0.05; **, Kruskal-Wallis, P < 0.01; ***, Kruskal-Wallis, P < 0.001. Symbols represent individual animals; horizontal dashed lines represent the limit of detection.