Skip to main content
. 2015 Apr 22;89(13):6792–6804. doi: 10.1128/JVI.00351-15

FIG 3.

FIG 3

HCMV infection differentially affects subcellular levels of NICD1 and Jag1 proteins in NPCs. Cellular fractions of membrane (Mem), cytosolic (Cyt), and nuclear (Nuc) proteins were prepared from mock-infected (M) and virus-infected (V) NPCs at 48 hpi and analyzed for NICD1 and Jag1 levels by Western blotting. Na+/K+-ATPase, GAPDH, and histone H4 served as loading controls for membrane, cytosolic, and nucleus proteins, respectively. (A) Western blot analysis of NICD1 and Jag1 levels. (B) Normal cellular distributions of NICD1 and Jag1. Western blotting results were quantitated densitometrically and used to calculate relative subcellular distributions of NICD1 and Jag1 in uninfected NPCs. Data are presented as means ± 1 standard deviation (SD) from three independent experiments. (C) Analysis of the differences in degradation of NICD1 and Jag1 in different cell fractions. Densitometric data were used to calculate the impact of viral infection on NICD1 and Jag1 levels in each cell fraction and normalized to the levels of mock-infected cells, which were set as a value of 1.0. Data are presented as means ± 1 SD from three independent experiments.