FIG 7.
pp71 induces the degradation of NICD1 and Jag1 via the proteasome in NPCs. NPCs were seeded at a density of 5 × 106 cells/dish. pCDH-GFP, pCDH-flag-UL26, pCDH-pp65, or pCDH-pp71 was transfected by nucleofection into NPCs that express Notch1/NICD1 and Jag1 endogenously. Cells were collected at 48 hpt. NICD1, Jag1, FLAG, pp65, and pp71 protein levels were analyzed by Western blotting. (A) Effects of UL26, pp65, and pp71 on endogenous NICD1 and Jag1. (B) MG132 treatment inhibits endogenous NICD1 and Jag1 degradation induced by pp71. pCDH-pp65 and pCDH-pp71 were transfected into NPCs. Cells were treated with MG132 for 20 h and collected at 48 hpt. NICD1, Jag1, pp65, and pp71 protein levels were analyzed by Western blotting.