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. 2015 Apr 15;89(13):6595–6607. doi: 10.1128/JVI.00009-15

FIG 3.

FIG 3

Viral growth kinetics of recombinant PICV NP RNase mutants in vitro. (A) Plaques formed in Vero cells by the respective viruses. (B) Viral growth kinetics in IFN-defective Vero and IFN-competent A549 cells. Cells were infected with the respective viruses at an MOI of 0.01. At different times postinfection, virus titers in the supernatants were quantified by plaque assay. The results shown are the averages for three independent experiments. (C) Viral growth kinetics in Vero and A549 cells in the presence of exogenously added IFN-β. Cells were infected with the respective viruses at an MOI of 0.01 for 1 h. After a PBS wash, fresh medium containing 100 IU of human IFN-β was added. Viral titers in the supernatants at different times postinfection were quantified by plaque assay. Each sample was compared to the rP18 group by statistical analysis using the Student t test. *, P < 0.05; **, P < 0.01.