FIG 8.

Host cell IκB expression levels affect HIV-1 latency establishment. On the kinase antibody arrays, IκBa was detected as the highest altered spot in latently HIV-1 infected CA5 T cells. To test for a possible influence of IκB on HIV-1 latency control, we retrovirally transduced J2574 GFP reporter T cells to express IκB. (A) Following infection of the parental J2574 cells and J2574-IκB cells over a wide MOI range, we compared the levels of HIV-1 expression in infected J2574 cells and J2574-IκB cells, under baseline and PMA-activated conditions, to demonstrate that IκB is overexpressed and physiologically active in J2574-IκB cells and that it reduces HIV-1 expression, measured using GFP mean channel fluorescence as a surrogate marker. (B) The infection levels of J2574 cells and J2574-IκB cells as a function of viral input were determined on day 3 postinfection. (C) The establishment of a silent infection reservoir in the paired infection cultures was compared on day 4 postinfection. (D) The establishment of a stable latent HIV-1 reservoir in the paired infection cultures was compared on day 14 postinfection. Each data set represents a summary of results for 30 paired infection cultures generated at different MOIs.