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Effect of Rad50 knockdown on AAV replication in the presence of AdV. (A) Level of Rad50 and Mre11 proteins in HeLa cells infected with either wt AdV (MOI, 50 IP/cell) or wt HSV-1 (MOI, 5 PFU/cell) at 48 h and 24 h p.i., respectively. (B) HeLa shRad50 and HeLa shLuc cells were coinfected with wt AAV (MOI, 103 GP/cell) and wt AdV for 48 h, and AAV DNA was quantified by qPCR. Results are expressed as the ratio of rep to β-globin genes (means and SD).
