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. 2015 Apr 22;89(13):6824–6834. doi: 10.1128/JVI.00171-15

FIG 4.

FIG 4

Effect of the rep gene sequences on AAV genome replication in MRN-negative or -positive cells. (A) Structure of the wt and recombinant AAV genomes used. The AAVtCR genome is composed of the p5 promoter and the rep ORF fused at its N terminus to the TAP-Cherry sequences (14). The AAVp5GFP vector contains the entire p5 promoter (37). (B) HeLa shRad50 and HeLa shLuc cells were coinfected with AAVtCR (MOI, 103 GP/cell) and either HSVΔPol or wt HSV (MOI, 5 PFU/cell). DNA extracted 20 h p.i. was analyzed by qPCR using rep-specific primers. *, P < 0.05. (C) HeLa shRad50 and HeLa shLuc cells were coinfected with either AAVGFP or AAVp5GFP (MOI, 103 GP/cell) and HSVΔPol (MOI, 5 PFU/cell). Rep activity was provided either by transfection of pRep or coinfection with wt AAV. GFP DNA levels were measured by qPCR.