TABLE 2.

Host factor effects on recombination frequencies in CMV strain Fny

Virus	Plant	Total no. of clones	No. of clones with restriction profile showna				Recombination frequency (%)
			BamHI	NcoI	No site	Two-site
F1F2F3 control	1	112	47	65	0	0	0
	2	108	67	41	0	0	0
	3	105	55	50	0	0	0
							Mean, 0
F1F2F3 in N. benthamiana	1	100	55	43	1	1	2.0
	2	124	88	34	2	0	1.6
	3	102	52	48	1	1	1.9
							Mean, 1.83

^aIn the control experiments, individual infections with each mutant were used to create a mixture in vitro, prior to RT-PCR and RFLP analysis. In the experimental samples (N. benthamiana), the plants were inoculated with a mixture of transcripts from the two mutants.