. 2015 Apr 22;89(13):6817–6823. doi: 10.1128/JVI.00040-15

TABLE 4.

CMV strain LS recombination frequencies calculated using various combinations of RNA 3 mutants

Virus	Expt or plant	Total no. of clones	No. of clones with restriction profile shown^a				Recombination frequency (%)^b
L₁L₂L₃ control	Expt		SpeI	BglII	No site	Two-site
	1	115	50	65	0	0	0
	2	103	55	48	0	0	0
	3	108	48	60	0	0	0
							Mean, 0
L₁L₂L₃	Plant		SpeI	BglII	No site	Two-site
	1	104	74	0	30	0	28.80
	2	110	78	0	32	0	29.09
	3	101	74	0	27	0	26.73
							Mean, 28.20 A^b
L₁L₂L₃	Plant		SpeI	NsiI	No site	Two-site
	1	105	86	0	19	0	18.00
	2	113	92	0	21	0	18.50
	3	102	86	0	16	0	15.68
							Mean, 17.39 B
L₁L₂L₃	Plant		SpeI	SacII	No site	Two-site
	1	101	81	0	20	0	19.80
	2	112	89	0	23	0	20.53
	3	111	89	0	22	0	19.80
							Mean, 20.04 C

In the control experiments, individual infections with each mutant were used to create a mixture in vitro, prior to RT-PCR and RFLP analysis. In the experimental samples, the plants were inoculated with a mixture of transcripts from the two mutants.

Differences between recombination frequencies were determined by ANOVA (P < 0.05). Statistically significant differences are marked with different capital letters next to the recombination frequencies.