TABLE 5.
Virus | Plant no. | Total no. of clones | No. of clones with restriction profile shown |
Recombination frequency (%)b | No. of deletion mutants | Deletion frequency (%) | |||
---|---|---|---|---|---|---|---|---|---|
SpeI | BglII | No site | Two-site | ||||||
L1L2L3a | 1 | 104 | 74 | 0 | 30 | 0 | 28.80 | 0 | 0 |
2 | 110 | 78 | 0 | 32 | 0 | 29.09 | 0 | 0 | |
3 | 101 | 74 | 0 | 27 | 0 | 26.73 | 0 | 0 | |
Mean, 28.20 A | Mean, 0 | ||||||||
L1F2L3 | 1 | 112 | 0 | 101 | 3 | 0 | 2.67 | 8 | 7.14 |
2 | 102 | 0 | 93 | 2 | 0 | 1.96 | 7 | 6.86 | |
3 | 118 | 0 | 105 | 3 | 0 | 2.54 | 10 | 8.47 | |
Mean, 2.39 B | Mean, 7.53 | ||||||||
F1F2L3 | 1 | 101 | 0 | 99 | 2 | 0 | 1.98 | 0 | 0 |
2 | 105 | 0 | 103 | 2 | 0 | 1.90 | 0 | 0 | |
3 | 114 | 0 | 111 | 3 | 0 | 2.63 | 0 | 0 | |
Mean, 2.17 B | Mean, 0 | ||||||||
F1L2L3 | 1 | 113 | 96 | 0 | 17 | 0 | 15.04 | 0 | 0 |
2 | 100 | 86 | 0 | 14 | 0 | 14.00 | 0 | 0 | |
3 | 125 | 104 | 0 | 21 | 0 | 16.80 | 0 | 0 | |
Mean, 15.28 C | Mean, 0 | ||||||||
L1F2aLS2bL3 | 1 | 100 | 0 | 98 | 0 | 0 | 0 | 2 | 2 |
2 | 103 | 0 | 102 | 1 | 0 | 0.90 | 0 | 0 | |
3 | 106 | 0 | 106 | 0 | 0 | 0 | 0 | 0 | |
Mean, 0.30 D | Mean, 0.65 |
Data from Table 4.
Differences between recombination frequencies were determined by ANOVA (P < 0.05). Statistically significant differences are marked with different capital letters next to the recombination frequencies.