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. 2015 Apr 15;89(13):6746–6760. doi: 10.1128/JVI.00423-15

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FIG 1 — Alignment of mosquito-borne flavivirus NS4B. Multiple-sequence alignment of flaviviral NS4B sequences was performed using Clustal Omega software. The corresponding amino acids of transmembrane helices of DENV2 are indicated above the DENV2 sequence. An asterisk represents a fully conserved residue, a colon represents strong similarity, and a period represents weak similarity among the residues according to the parameters of Clustal Omega. The putative N-glycosylation sites from NetNGlyc prediction software are shown in white on a black background. DENV2, strain PL046, Asn-58 (NSSV), Asn-62 (NVSL), Asn-214 (NTTI), and Asn-242 (NTTN). DENV1, strain 45AZ5 vaccine, Asn-59 (NTTA), Asn-63 (NISL), and Asn-215 (NTTI). DENV3, strain H87, Asn-58 (NSTA), Asn-62 (NVSL), and Asn-214 (NTTI). DENV4, GenBank accession number AF326825.1, Asn-55 (NTSA), Asn-59 (NLSL), and Asn-211(NTTI). WNV, West Nile virus, strain NY99, Asn-26 (NFSM), Asn-66 (NTSL), and Asn-219 (NATT). YFV, yellow fever virus, strain 17D vaccine, Asn-64 (NLSL), Asn-90 (NISV), and Asn-211(NTSL). JEV, Japanese encephalitis virus, strain JaOArS982, Asn-191 (NVTT) and Asn-219 (NSTT).