FIG 3.

Reproducibility and cluster analysis. (A) Ribosome profiling with cycloheximide (Ribo-CHX). Read counts were mapped to the annotated ORFs of VACV from two independent experiments at 2, 4, and 8 h postinfection. Each dot represents the read count for an individual mRNA. The correlation coefficients (r) are noted on each plot. (B) Cluster analysis of early gene expression. Cluster analysis was performed on the normalized read counts of ribosome footprints for each early gene at 2, 4, and 8 h after infection. Line plots of the read counts corresponding to three clusters are shown.