TABLE 1.
Highly translated viral mRNAs and their encoded protein function categories at 2, 4, and 8 h post-VACV infection
Time of expression and VACV WR ORFa | VACV Copenhagen ORF | Temporal expressionb | Functional category | No. of readsc |
---|---|---|---|---|
2 h | ||||
009/210 | C11R | E | Host interaction | 21,247,140 |
103 | H5R | E/Ld | Transcription | 19,521,388 |
18 | E | Unknown | 18,505,965 | |
59 | E3L | E | Host interaction | 16,992,412 |
72 | I3L | E | DNA replication | 12,105,947 |
89 | L2R | E | Virion association | 8,753,364 |
29 | N2L | E | Host interaction | 6,987,681 |
200 | B19R | E | Host interaction | 6,446,599 |
172 | A46R | E | Host interaction | 5,931,269 |
41 | F2L | E | DNA replication | 5,731,347 |
4 h | ||||
70 | I1L | I | Virion association | 31,221,695 |
133 | A14L | L | Virion association | 27,674,145 |
56 | F17R | L | Virion association | 18,595,167 |
131 | A12L | I | Virion association | 17,138,138 |
103 | H5R | E/L | Transcription | 15,370,215 |
132 | A13L | L | Virion association | 12,605,197 |
139 | A19L | I | Virion association | 11,987,935 |
137 | A17L | L | Virion association | 11,388,463 |
28 | N1L | E/Ld | Host interaction | 11,342,780 |
157 | A34R | I | Virion association | 9,677,241 |
8 h | ||||
133 | A14L | L | Virion association | 32,690,917 |
70 | I1L | I | Virion association | 24,134,410 |
167 | A42R | I | Virion association | 18,693,389 |
56 | F17R | L | Virion association | 17,013,444 |
131 | A12L | I | Virion association | 16,613,835 |
28 | N1L | E/L | Host interaction | 14,260,755 |
103 | H5R | E/L | Transcription | 13,848,524 |
132 | A13L | L | Virion association | 12,915,930 |
139 | A19L | I | Virion association | 12,450,056 |
137 | A17L | L | Virion association | 10,427,057 |
For each time period post-VACV infection, the top 10 most highly translated mRNAs are listed.
The mRNAs are listed in rank order in terms of the normalized number of reads mapped to the ORF.
The L-stage promoter was not distinguished from the intermediate one.