FIG 2.

GFP-positive CCR5 knockdown sorted CD34⁺ HSPCs produced mice with “pure” populations of transduced cells in vivo. (a) FACS plots showing the percent human engraftment of CD45⁺ (percentage of live cells) and GFP-positive CD45⁺ (percentage of human CD45) cells for representative control-transduced, FACS-sorted R5 knockdown, and FACS-sorted negative mice before HIV infection. (b) FACS plots showing the percentage CD4⁺ T cells (percent CD3⁺), CD8⁺ (percent CD3⁺), and GFP-positive CD4⁺ T cells (percent CD4⁺) in the peripheral blood before HIV infection of the same mice as in panel a. For all cell subset analyses, the subgating was done as follows: total live cells and CD45⁺, CD3⁺, CD4⁺, and CD8⁺ cells. Mean fluorescence intensity (MFI) for GFP is indicated.